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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which ends up in chromosome instability, and so could lead to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical position in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two supplemental splice variants have been documented but detailed nucleotide sequence facts just isn't however out there.
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Blocking peptides are peptides that bind specifically towards the target antibody and block antibody binding. These peptide typically consists of the epitope regarded with the antibody.
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This tutorial outlines further more attainable optimization of mobile and immunoassay parameters to be certain the very best results are...
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This information outlines further possible optimization of mobile and immunoassay parameters to be certain the very best effects are...
Antibodies bound to the blocking peptide now not bind on the epitope within the target protein. By evaluating the staining from the blocked antibody vs . the more info antibody on your own, you can see which staining is distinct.
Concentrate on Facts Cyclin E belongs to your highly conserved cyclin family members, whose associates are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is also degraded as cells development by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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